Polyclonal Anti-HIF-1α
Technical Data
- Product #
- AG10001
- Product name
- Polyclonal HIF-1α
- Host
- Rabbit
- Immunogen
- Human HIF-1a peptide
- Cross-Reactivity
- Human, Mouse, Hamster
- Available Size
- 50µl; contains Thimerosal (0.01%)
Applications
- Immunohistochemistry
- Yes
- Western Blot
- Yes | dilute at 1:3000
- Immunofluorescence
- Yes | dilute at 1:3000
Handling Requirements
- Storage
- Store at -20°C. Avoid repeated freezing and thawing.
Notes
This antibody reacts with human HIF-1a (hypoxic conditions) in western blotting and can also be used to study HIF-1a in mouse and hamster cells.
References
This anti-HIF-1a antibody has been validated by siRNA as described in: Berra E, Benizri E, Ginouves A, Volmat V, Roux D, Pouyssegur J. HIF prolyl-hydroxylase 2 is the key oxygen sensor setting low steady-state levels of HIF-1a in normoxia. The EMBO Journal 22(16): 4082-4090, 2003.
Pricing
- Cost
- $250.00
- Shipping & Handling
- Fedex or UPS cost + $10 dry ice fee
Western Blot
anti-HIF-1α rabbit
polyclonal antiserum
Immunofluorescence
HIF-1α induction by hypoxia (1%) in MCF7 cells
