Polyclonal Antibody Development
Why Precision Antibody?
We generate high-affinity specific mouse polyclonal antibodies to target antigens in less than 3 weeks. Our polyclonal antibodies have a titer range of 1:10,000 to 1:100,000. A typical Precision Antibody™ project generates approximately 2 ml serum with titer exceeding 1:50,000. That’s 100L worth of serum at working dilutions! We can produce expandable polyclonals with consistent performance.
Types of Projects
Our Precision Antibody™ team has developed polyclonal antibodies to targets including parasites, cancer-specific surface antigens, GPCRs, hormones, growth factors and their receptors, glycoproteins, peptides, proteins and their modification sites (mutation site-specific, phosphorylation site-specific, cleavage site-specific, etc), and other antigens such as small-molecule compounds and DNA. We have created antibodies for various applications, such as functional assays, antibodies for immunoprecipitation, IHC, IF, flow cytometry, western blot, and EIA. Please talk to our experienced researchers regarding your project and application
| Protocol | Basic Poly | Poly Plus | Platinum Poly | Platinum Poly Plus |
|---|---|---|---|---|
| Price | $1,000 | $1,500 | $2,500 | $3,000 |
| Initial Consultation: Antigen Selection, Design & Screening |  | | | |
| Immunization of Mice | | | | |
| Tail bleed & Serum screening by EIA | | | | |
| Email data to customer for evaluation & feedback | | | | |
| Final Bleed & Amount | 2 ml | 2 ml | 15 ml | 15 ml |
| Protein A or G Purification (affinity upon request) | | | ||
| SDS-Page Analysis | | |
Polyclonal Antibody Pricing
- Deliverables:
- With Standard Polyclonal Package, we will deliver approximately 2 ml serum with guaranteed titer at least 1:10,000 (Range 1:10,000 to 1:100,000). With Platinum Polyclonal Package, we will deliver at least 15 ml with guaranteed titer at least 1:10,000 (Range 1:10,000 to 1:100,000).
- Project Timeline:
- 3 week or less for the Standard Polyclonal Package. 4 weeks for the Platinum package.
Greatest Benefit for using Precision Antibody
The greatest benefit about using Precision Antibody for polyclonal projects is our speed, titer and and flexibility. We deliver high titered mouse polyclonal antisera in 3 weeks or less. In addition, our customers have the choice to pursue a monoclonal antibody at the end of the polyclonal project. That’s right! If you like the polyclonal results, you have the choice to bring your project one step forward. If you decide to go for mAB development, we will give you a $500 discount on the monoclonal project.
Mouse Polyclonal vs Rabbit Polyclonal
When it comes to polyclonal antibody development, most customers think of rabbit or even larger animals. At Precision Antibody, we believe mouse polyclonal antibody is a viable and preferred option for many of our customers especially for those who need rapid quality antibody development. See below for easy comparison.
| Precision Antibody | Competitors | |
|---|---|---|
| Antigen Requirement | 0.5 mg | 4.0 mg |
| Precision Antibody has the advantage of requiring less antigen for immunization. This is important when dealing with difficult or costly antigens. | ||
| Animals Used | Mice | Rabbits |
| Precision Antibody is OLAW friendly. | ||
| Timeline | 21 days | 62 days |
| Precision Antibody provides rapid antibody development service that meets your deadline. | ||
| Final Serum Amount | 2 ml | 100 ml |
| Precision Antibody offers Platinum Service where we will delver 15 ml of high titer polyclonal antibodies. That's equivalent to 100 ml of rabbit antisera at 1:10,000 titer. | ||
| Titer (Average) | 1:50,000 | 1:10,000 |
| Precision Antibody delivers high titered antisera | ||
| Working Solution | 100 L | 1,000 L |
| If you need larger volumes, Precision Antibody's Platinum Service can provide volumes of working antibody solution, that is equivalent to rabbit polyclonal, in a shorter period of time. | ||
To contact our staff for your next antibody project:
Call us at 410-884-4100, email orders@precisionantibody.com, or try our online quote request form. We provide free consultation and an exact price quote for all antibody projects.