In vitro antibody production
In Vitro Antibody Production
Precision Antibody™ has broad experience in small scale (mg to 10 mg) to large scale (100 mg to g) production of monoclonal antibodies from hybridoma cells, CHO cells, and HEK293 cells.
The antibody production rate differs from clone to clone. Precision Antibody™ offers two types of production services:
I. Production based on the volume of culture medium: 10 ml ~ 40 L
II. Production based on the amount of antibody: 50 mg ~ grams
In Vivo Production
Precision Antibody™ also has an AAALAC accredited animal facility for ascites production. Occasionally, we encounter hybridoma cells that do not produce monoclonal antibodies in in vitro systems. Ascites production can be achieved using nude mice/rats or syngeneic host mice as an alternative method to produce such monoclonal antibodies. Ascites production often yields higher concentrations of monoclonal antibody. We prefer to use nude athymic mice for ascites production to eliminate the contamination of endogenous mouse IgG.
Summary of Production Methods We Support:
- Static Culture – up to 200 ml
- Roller bottle culture
- Gas permeable culture bags 50ml – 2 L
- CelLine disposable flasks
- FiberCell Hollow fiber system
- Shaker flask 3.5-1 L
- BioFlo 320 bioreactor system 5 to 40 L
- Ascites nude mice production
Antibody Purification
Precision Antibody™ offers several different methods for antibody production.
I. Affinity purification using Protein A, G, A/G, L, or Mellon.
II. Affinity purification using antigen affinity column.
III. Classical IEC and SEC purification.
We also offer additional services including endotoxin testing, sterility testing, isoelectric focusing, aggregation testing, and cell banking.
Documentation:
Certificate of Analysis (SDS-PAGE, EIA, and Ab quant-based on A280) can be provided.
We provide free consultation and an exact price quote for all antibody projects.
Disclaimer:
All clones developed are the customer’s property.
Precision Antibody does not retain any ownership.
We are happy to exchange CDA, NDA, & MSA.
