Immunization

• Initial consultation: antigen selection, design and immunization strategy.
• Immunization of 3-5 mice.
• Serum screening by EIA or other assay per project strategy.

3 weeks

Fusion & Cloning

• Fusion using mouse with highest specific antibody titer.
• Cloning of hybridoma.
• Standard project: A minimum of 500 clones are picked for screening.

2 weeks

Screening

• Initial and Confirmatory ELISA screening or alternative screening based on project strategy.
• Confirmatory Data will be sent for review.
• Additional charges may apply if multiple antigen screening is required.

1 week

Cell Culture & Sup Prep

• Expand the clones and prepare culture supernatant for confirmed positive clones.
• Cell culture expansion, supernatant preparation, cryopreservation and Ig Quantitation
• Supernatants sent to customer for final screening in application specific assay.

1.5 weeks

Octet Based Screening

• Octet Based Scouting Affinity & Epitope Binning (mAb pairing)

1-2 weeks

Clone & Antibody Delivery

• Clones selected by customer will be expanded.
• 3 Vials per clone will be sent.
• Storage of 5 selected clones (3 vials per clone) with storage in LN (Taylor Wharton LABS40K) for up to 3 years.

1-2 weeks

Small Scale Production for Clone Validation and Selection*

• Bag Production (up to 5mgs)
• Protein A or Protein G affinity purification
• Certificate of Analysis, includes binding ELISA (Target Ag).
• IgG isotyping and SDS-PAGE.

4-6 weeks