Anti-idiotype antibodies are categorized into two types: anti-paratope (competes with the antigen for binding to DA) and anti-idiotope (does not interfere with antigen binding). When generating anti-idiotype antibodies, it’s essential to differentiate between anti-paratope and anti-idiotope antibodies. Anti-paratope antibodies are unable to bind to antigen-bound DA, making them unsuitable for detecting total circulating DA.

Precision Antibody™ utilizes a proprietary immunization protocol, Focus Technology, that significantly enhances anti-idiotype antibody generation. With well over 200 successful antiidiotype projects delivered to both large and small biotech and pharmaceutical clients, our success rate in producing anti-idiotype antibodies with desired performance characteristics is 100%.

Our clients typically request antibody pairs for developing sandwich ELISAs to measure circulating DAs. Precision Antibody™ selects high-affinity, low-off-rate capture and detection antibody pairs with minimal matrix effects (serum interference) for optimal PK study performance.