Humanization of IgG Antibodies
Humanization of IgG Antibodies – 5x5 Method
Precision Antibody™ offers a robust humanization process using the 5×5 Method, which generates 25 unique combinations of humanized antibodies. This is done by applying five distinct techniques covering CDR and Framework Regions of the heavy and light chain variable regions. Each method serves a specific purpose to optimize antibody humanization, antigen binding, and reduce immunogenicity:
- CDR-Grafting
- Veneering
- Frankensteining
- SDR Transfer
- Abbreviated CDR Grafting
The services include:
- Humanization and optimization of humanized sequence.
- Synthesis of the resulting Humanized VH VL genes.
- Expression of 25 Humanized Antibodies.
- Verification of antigen binding parameters by EIA and Octet.
- The selected sequence can be expressed using desired human IgG subclass.
- Transient expression or Stable cell line development.
- Production & Purification of Humanized antibodies.
Disclaimer:
All clones developed are the customer’s property.
Precision Antibody™ does not retain any ownership.
We are happy to exchange CDA, NDA, & MSA.
Benefits
- Dedicated Project Manager
- AAALAC Accredited/USA Lab
- Timeline – Rapid Timeline 60 days
- Milestone Based Invoicing/IP Free
Contact us for your next Humanization of IgG Antibodies project
For inquiries about your next Humanization of IgG Antibodies project, please call us, email us, or use our online quote request form for a free consultation and pricing.
- 410-884-4100
- 410-884-1607
- orders@precisionantibody.com
- 9130 Red Branch Road, Suite X, Columbia, MD 21045
- Mon-Fri 9:00AM – 5:00PM
