Recombinant IgG Expression & Production
Recombinant Antibody Expression/Production
Precision Antibody™ offers antibody gene cloning and the construction of expression vectors for transient antibody expression or the development of stable cell lines expressing recombinant antibodies. These antibodies can be produced in either CHO or HEK293 cells. Precision Antibody™ can scale production from milligrams to grams, using a culture system tailored to the required production amounts.
- Planning for a long-term production of monoclonal antibodies.
- Hybridoma producing the antibody of interest is a low producer.
- A small amount of purified antibody is available but lost the hybridoma.
- Isolated antibody of interest from a phage library or B cells.
- Need to produce humanized antibody.
- Need cell line development for fully human or humanized monoclonal antibody.
Precision Antibody™
Can help you clone and generate expression vector to be used for recombinant antibody production in CHO and HEK293 cells.
For fully human monoclonal antibody producing hybridoma cells, we can sequence the variable region of H and L chains (VH & VL) using MiSeq.
For mouse and rat monoclonal antibodies, we can amplify the variable region gene by PCR using redundant primer sets.
Optimized H and L chain genes are synthesized and incorporated into appropriate expression vector system. A small amount of recombinant antibody is produced and characterized to confirm their specificity and biophysical characteristics (KD, ka, & kd).
General Process:
- Sequence analyses of VH & VL
- Optimize the VH & VL
- VH gene syntheses.
- Cloning of VH into IgG vector and sequence confirmation.
- L chain gene syntheses, cloning into expression vector and sequence confirmation.
- Transient production of small amounts of IgG.
- EIA and Octet based confirmation of antigen specificity and binding parameters (KD, ka, & kd).
- Ultra-low endotoxin plasmid production/purification.
- Transient IgG production in CHO or HEK293 cells or stable cell line development.
Disclaimer:
All clones developed are the customer’s property.
Precision Antibody™ does not retain any ownership.
We are happy to exchange CDA, NDA, & MSA.
Contact us for your next Recombinant IgG Expression & Production project
For inquiries about your next Recombinant IgG Expression & Production project, please call us, email us, or use our online quote request form for a free consultation and pricing.
- 410-884-4100
- 410-884-1607
- orders@precisionantibody.com
- 9130 Red Branch Road, Suite X, Columbia, MD 21045
- Mon-Fri 9:00AM – 5:00PM
Benefits
- Dedicated Project Manager
- AAALAC Accredited/USA Lab
- Timeline – Rapid Timeline 60 days
- Milestone Based Invoicing/IP Free
