Screening & Functional Assays

Antibodies play a crucial role across diagnostic, biomarker, and therapeutic fields, with each application requiring precise development, verification, and validation. At Precision Antibody™, we take this responsibility seriously by integrating specialized assays directly into the antibody development process. This ensures that our customers receive functional antibodies that work effectively in their intended applications.

From the outset, our team collaborates closely with customers to understand the final application of the antibody. We incorporate antigen design strategies and carefully select appropriate screening assays as part of the statement of work, tailored to meet project goals.

In addition to development, we offer specialized assays to evaluate the efficacy of selected antibodies in pre-clinical studies.

Specialized Assays We Offer

Internalization

For the development of therapeutic antibody drug conjugate (ADC) against internalizing targets such as receptors or cell surface proteins, it is essential to select antibodies that induce target internalization. Precision Antibody™ offers fluorescence- based identification of antibodies that upon binding facilitate cell surface target internalization. An initial assay can be carried out using hybridoma culture sups. Once internalizing antibodies are identified, small scale productions and purifications of internalizing antibodies will be performed, and a time course and a dose response of internalization will be carried out using purified antibodies. Octet-based scouting assays can be performed to select antibodies with desired affinity parameters.

Neutralization

Precision Antibody™ offers binding inhibition assay to identify antibodies that block ligand binding to its receptor. Binding inhibition assay can also be used to identify antibodies that block protein-protein interactions, signal transduction, receptor dimerization, substrate binding to the catalytic domain of an enzyme, or cell adhesion. Neutralization Assays can be performed by Octet, ELISA, or by functional assays. Octet-based scouting assays can be performed to select antibodies with desired affinity parameters.

Flow Based Assays

Antibodies generated against the extracellular domains of plasma membrane-associated targets can be evaluated by flow cytometry-based binding assay for screening and validation using a high throughput Intellicyt flow cytometer. Antibodies for flow analyses must recognize the natural conformation of the cell surface antigens. The screening and validation of antibodies using flow cytometry assay require both antigen-positive and negative cells. The Oncology Panel Profiler can help identify candidate cells that will be used in the assay. The flow cytometry binding assay can be used with hybridoma supernatants as part of the hybridoma screening process as well as with purified antibodies for the validation of selected antibodies binding to cell surface proteins.

Biological Assays

To ensure that selected therapeutic antibodies are biologically effective for their intended applications, a series of in vitro and in vivo biological assays which can also be incorporated into Preclinical Efficacy Testing. This section outlines various in vitro and in vivo assays designed for this purpose. The Oncology Panel Profiler can also be used to select cancer cell lines for in vitro and in vivo biological assays, enhancing the accuracy of preclinical testing.

Disclaimer:

All clones developed are the customer’s property.
Precision Antibody™ does not retain any ownership.
We are happy to exchange CDA, NDA, & MSA.

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